| 產(chǎn)品名稱 |
pBCRF1 |
| 商品貨號(hào) |
B239932 |
| Designations |
pBCRF1 |
| GenBank Number |
V01555
|
| Species |
Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus |
| Depositors |
DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Vector |
Name of vector: pcDSRalpha
Construct size (kb): 4.30
Type of vector: plasmid
Construction: pcDV1, HTLV-1 LTR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
centromere: c
marker(s): ampR
promoter: SV40/HTLV-1 LTR
replicon: pMB1, SV40
|
| Insert |
Genome: Epstein-Barr virus
Gene symbol: BCRF1
Gene name: open reading frame BCRF1
Contains complete coding sequence?: U
Type of DNA: genomic
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: V01555DNA: genomic
Insert lengths(kb): 0.70
Gene product: open reading frame BCRF1 [BCRF1] |
| Insert Size (kb) |
0.70 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 0.7; EcoRI--3.8, 0.5; XhoI--3.6, 0.7. EcoRI will also excise the insert. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| Classification |
Herpesviridae, Lymphocryptovirus |
| References |
Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510
Hsu DH, et al. Expression of interleukin-10 activity by Epstein-Barr virus protein BCRF1. Science 250: 830-832, 1990. PubMed: 2173142
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
