| 產(chǎn)品名稱 |
c8083 |
| 商品貨號(hào) |
B237857 |
| Designations |
c8083 |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
L Riles, MV Olson |
| Vector |
Construct size (kb): 50.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: TOP3
Gene name: topoisomerase (DNA) III
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: EST1
Gene name: telomere elongation protein (probable telomerase)
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: CDC42
Gene name: cell division cycle 42 (GTP-binding protein, 25kD)
Contains complete coding sequence?: Y
Chromosome: XII
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 43.5
Cross references: Insert lengths(kb): 43.5 Gene product: cell division cycle 42 (GTP-binding protein, 25kD) [EST1] |
| Insert Size (kb) |
43.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen, uncharacterized |
| Comments |
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
