国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Hs 184.T
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Hs 184.T
Hs 184.T
規(guī)格:
貨期:
編號:B164656
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Hs 184.T
商品貨號 B164656
Organism Homo sapiens, human
Tissue bone
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease osteosarcoma
Age 21 years adult
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data
male
Caucasian
21 years adult
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
THO1: 9,9.3
TPOX: 12
vWA: 15,16
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
临潭县| 固原市| 米泉市| 安吉县| 扬中市| 广德县| 涿鹿县| 临邑县| 晋宁县| 嘉荫县| 绍兴市| 万山特区| 顺平县| 县级市| 文水县| 聂荣县| 平南县| 申扎县| 报价| 宜良县| 安龙县| 东台市| 吕梁市| 刚察县| 阆中市| 淮安市| 阳城县| 胶南市| 通州市| 多伦县| 朔州市| 黑山县| 榆社县| 长治市| 青冈县| 河南省| 滨海县| 北碚区| 富源县| 三门峡市| 祥云县|