国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > HP6050
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
HP6050
HP6050
規(guī)格:
貨期:
編號(hào):B164640
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HP6050
商品貨號(hào) B164640
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with hinge region epitopes that are different from those reactive with HP6047 (ATCC CRL-1774).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified human myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human IgG3 hinge region
Cellular Products
immunoglobulin; monoclonal antibody; against human IgG3 hinge region
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with purified human myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with hinge region epitopes that are different from those reactive with HP6047 (ATCC CRL-1774).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • 0.05mM 2-Mercaptoethanol (final conc.)
  • 10%fetal bovine serum (final conc.)
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
    • Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
    Medium Renewal: Every 2 to 3 days depending on the cell density.
    Cryopreservation

    Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype IgG1
    Name of Depositor CB Reimer
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201

    Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
    揭东县| 桂平市| 古蔺县| 东平县| 巫山县| 溧水县| 卓尼县| 鄂托克前旗| 平罗县| 德清县| 温泉县| 林周县| 怀安县| 浮梁县| 台南市| 彝良县| 封丘县| 抚州市| 安塞县| 时尚| 包头市| 东台市| 民权县| 全南县| 富顺县| 高碑店市| 竹溪县| 关岭| 玉林市| 泉州市| 兴仁县| 尚义县| 大竹县| 岳西县| 荔浦县| 江津市| 南投市| 佛学| 四子王旗| 灌云县| 湖口县|