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The line was established from normal skin from the abdomen.

female

Caucasian

46 years

The cells senesce after approximately 37 population doublings.

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Culture medium, 95%; DMSO, 5%