| 產(chǎn)品名稱 |
7D2-1.4.1.5 |
| 商品貨號 |
B163826 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with both mouse gamma 2a (IgG2a) and gamma 2b (IgG2b). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified mouse IgG2a and IgG2b. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse IgG2 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse IgG2 |
| Comments |
Animals were immunized with purified mouse IgG2a and IgG2b. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with both mouse gamma 2a (IgG2a) and gamma 2b (IgG2b). Tested and found negative for ectromelia virus (mousepox). A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is helpful in the initiation of a culture from a thawed ampule. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days These cells tend to attach strongly to the culture flask or dish. For the first few subcultures after thawing, it may be necessary to use a mild trypsin treatment to recover the attached cells. Afterward, the culture can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b; kappa light chain |
| Name of Depositor |
JF Kearney |
| Deposited As |
rat (B cell); mouse (myeloma) |
