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548
548
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編號:B163782
品牌:Mingzhoubio

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產(chǎn)品名稱 548
商品貨號 B163782
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Leukemia
Applications
The antibody reacts with the p12 protein of most MuLV.
The antibody reacts with infected cells after acetone or methanol fixation and immunoprecipitates p12.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells from a mouse recovering from splenomegaly induced by Moloney murine leukemia virus (MoMuLV) and Spleen Focus Forming virus (SFFV) were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against p12 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against p12 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Comments
Spleen cells from a mouse recovering from splenomegaly induced by Moloney murine leukemia virus (MoMuLV) and Spleen Focus Forming virus (SFFV) were fused with NS-1 myeloma cells.
The antibody reacts with the p12 protein of most MuLV.
The antibody reacts with infected cells after acetone or methanol fixation and immunoprecipitates p12.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor B Chesebro
Deposited As mouse (B cell); mouse (myeloma)
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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