| 產(chǎn)品名稱(chēng) |
Bunyamwera virus |
| 商品貨號(hào) |
B162833 |
| Classification |
Peribunyaviridae, Orthobunyavirus, Bunyamwera orthobunyavirus |
| Agent |
Bunyamwera virus |
| Strain |
Original |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1.0 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Derived by adaptation of mouse propagated product ATCC® VR-87™ to Vero C1008 cells (ATCC® CRL-1586™). ATCC® VR-87™ and ATCC® VR-1873™ have not been compared for sequence or infectivity. |
| Effect on Host |
CPE, refractile rounding and sloughing |
| Recommended Host |
Vero C1008 cells (ATCC® CRL-1586™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 3-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer. |
| Name of Depositor |
J Casals, The Rockefeller Foundation |
| Chain of Custody |
ATCC <-- J Casals |
| Source |
Aedes spp. Mosquitoes caught in Bunyamwera, an uninhabited area in Western Uganda, 1943 |
| Year of Origin |
1943 |
| References |
Smithburn KC, et al. A neurotropic virus isolated from Aedes mosquitos caught in the Semliki forest. Am J Trop Med Hyg 26: 189-208, 1946. PubMed: 21020339
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